POSTOPERATIVE CHANGES OF SPERM CHROMATIN HETEROGENEITY, USING ACRIDINE ORANGE STAINING, IN VARICOCELE PATIENTS
نویسندگان
چکیده
منابع مشابه
The effect of varicocele on sperm morphology and DNA maturity: does acridine orange staining facilitate diagnosis?
OBJECTIVE The aim of this study was to determine whether Acridine Orange (AO) can be used as a routine procedure in a physician's office to detect sperm deoxyribonucleic acid (DNA) fragmentation in patients with varicoceles. MATERIAL AND METHODS Forty-five patients with a mean age of 30.4 years, who had been diagnosed with varicoceles by physical examination and 30 healthy males with a mean a...
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Acanthamoeba keratitis is uncommon, but one of the most severe infectious diseases of the cornea. Delayed diagnosis or misdiagnosis as bacterial or herpes simplex keratitis leads to extensive corneal inflammation and profound visual loss. Therefore, accurate and rapid diagnosis of Acanthamoeba keratitis is essential for successful treatment and good prognosis. We evaluated the usefulness of acr...
متن کاملHistochemical observations on Mycoplasma after staining with acridine orange.
Mycoplasma colonies and Mycoplasma cells in preparations from infected milk and lymph nodes were observed for their fluorescent qualities after treatment with acridine orange. Mycoplasma colonies fluoresced brilliant red or red-orange. When treated after exposure to ribonuclease, the colonies fluoresced lime-green. There was no fluorescence when both ribonucleic acid and deoxyribonucleic acid w...
متن کاملAcridine Orange and Flow Cytometry: Which Is Better to Measure the Effect of Varicocele on Sperm DNA Integrity?
We evaluated the effect of varicocelectomy on semen parameters and levels of sperm DNA damage in infertile men. A total of 75 infertile men with varicocele and 40 fertile men (controls) were included in this study. Semen analysis and sperm DNA damage expressed as the DNA fragmentation index using acridine orange staining and chromatin condensation test by flow cytometry were assessed before and...
متن کاملP-13: Evaluation of Sperm Acrosin Activity and Chromatin Structure in Varicocele Patients Before and after Varicocelectomy
Background: Evaluation and comparison of total acrosin activity along with DNA integrity in the semen samples obtained from the varicocele individuals before and after the surgery and also in fertile individuals. Materials and Methods: Semen parameters, protamine deficiency, DNA fragmentation and Total acrosin activity were measured in patients with a clinical diagnosed varicocele of grade II o...
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ژورنال
عنوان ژورنال: Archives of Andrology
سال: 2006
ISSN: 0148-5016
DOI: 10.1080/01485010500428397